Purpose: The antibacterial activity of three endodontic sealers, a Ca (OH)2 based sealer (apexit) a Zoe based sealer (Drofill) and a resin based sealer (AH26) was assessed on the growth of an anaerobic bacteria (peprostreptococcus spp.) using the Agar diffusion test (ADT). Materials and Method: Thirty brain heart diffusion agar plates were incubated with peptostreptococcus anaerobics. Each plate was divided into two separate areas. In one area, 0.1ml droplet of a given fresh sealer and in the other area a dry material of the same sealer were placed, such that each plate had two areas with the same sealer in two forms of fresh and dry (setform). There were ten plates for each of the three sealers. The plates were placed into anaerobic jars and incubated at 37oC. After varying periods, zone of inhibition of bacterial growth were observed, measured and compared by t and Paired T-test.Results: There was no statistically significant differences between the activity of AH26 and drofill in the fresh form(P>0.05). However there were significant differences between (the two sealers in set form) (P<0.05) Drofill was more antibacterials in the set form than AH26. All sealers were more active when fresh than ages.Conclusion: It is likely that the eugenol in the Dorifill and the formaldehyde in the AH26 are responsible for their greater antimicrobial activity.

Introduction: After cleaning and shaping of the Root canal, to prevent recontamination and to ensure the success of Root canal therapy, it is necessary to fill Root canal space. Studies, so far, have not been able to identify a specific method as the best method of obturation. The present study was an attempt to introduce and compare different Root canal obturation techniques.Materials and Methods: In this review, using specific key words such as Root canal obturation, Root canal filling materials and gutta-percha, search was run in Pubmed, Cochrane and ISI Web of Science databases for high-quality articles published from 1960 to 2013.Results: There are several methods to obturate the Root canal system, designed based on the use of plastic materials, injectable materials, single cones, or compaction of gutta-percha. A brief review of each technique and its advantages and disadvantages were discussed. The reasons for choosing the cold lateral compaction technique as a frequently used and the selected technique by many dentists as well as academic centers were explained.Conclusion: Considering the advantages and disadvantages and long-term outcomes of Root canal obturation techniques, it seems cold lateral compaction is the selected Root canal filling technique in the majority of cases.